pgl3 basic 180 luciferase reporter plasmid (Addgene inc)
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Addgene inc
pgl3 basic 180 luciferase reporter plasmid
Pgl3 Basic 180 Luciferase Reporter Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 195 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pgl3-basic+plasmid/pGL3+(-BRE)+Luciferase+(Plasmid+%2345127)/10__1158_slash_0008___5472__can___25___2893-63-26-31
Average 95 stars, based on 195 article reviews
Pgl3 Basic 180 Luciferase Reporter Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 195 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pgl3-basic+plasmid/pGL3+(-BRE)+Luciferase+(Plasmid+%2345127)/10__1158_slash_0008___5472__can___25___2893-63-26-31
Average 95 stars, based on 195 article reviews
pgl3 basic 180 luciferase reporter plasmid - by Bioz Stars,
2026-10
95/100 stars
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Binding Assay:Article Title: Bmal1 Regulates Vascular Calcification via Non-Canonical Circadian Pathway Article Snippet: The aortic expression of Runx2 and SMA in arteries from the STZ-treated Bmal1 flox/flox and Bmal1 Δ/Δ mice were determined by immunofluorescent staining of consecutive 7-μm aortic sections using specific primary antibodies (Runx2, MBL Cat# D130–3 and SMA, Sigma Cat# A5228). .. To assess a direct regulation of Bmal1 on Runx2 transcription, a 1,974-bp mouse Runx2 promoter (mm10: chr17:44,735,972–44,737,945) containing putative Bmal1 binding sites was insert into the Article Title: Targeted protein O-GlcNAc reveals transcriptional functions for O-GlcNAc. Article Snippet: Technology Targeted protein O-GlcNAc reveals transcriptional functions for O-GlcNAc Plasmid Preparation:Article Title: Bmal1 Regulates Vascular Calcification via Non-Canonical Circadian Pathway Article Snippet: The aortic expression of Runx2 and SMA in arteries from the STZ-treated Bmal1 flox/flox and Bmal1 Δ/Δ mice were determined by immunofluorescent staining of consecutive 7-μm aortic sections using specific primary antibodies (Runx2, MBL Cat# D130–3 and SMA, Sigma Cat# A5228). .. To assess a direct regulation of Bmal1 on Runx2 transcription, a 1,974-bp mouse Runx2 promoter (mm10: chr17:44,735,972–44,737,945) containing putative Bmal1 binding sites was insert into the Article Title: TFEB coordinates autophagosome biogenesis and ribophagy during starvation via SQSTM1 Article Snippet: .. The promoter region of human SQSTM1 was amplified by PCR from the U2OS genome and cloned into the Article Title: The Interaction of CircESR1 and HNRNPAB Regulates Cell Cycle Transition of Breast Cancer Cell Article Snippet: .. For transcription factor mediated HNRNPAB expression, 0.2 μg Article Title: METTL3 Methylation Induces Decay of Endogenous Retroelement Transcripts to Promote Tumor Immune Evasion Article Snippet: The genes of SMYD1-5, 177 SETD7, SETD8 were inserted into pcDNA3.1(+) vector (RRID:Addgene_52535). .. The 178 SETD1A and E2F4 promoter region, spanning nucleotides −2000 to +100 relative to 179 the transcription start site (TSS), was PCR-amplified and ligated into the Construct:Article Title: Bmal1 Regulates Vascular Calcification via Non-Canonical Circadian Pathway Article Snippet: The aortic expression of Runx2 and SMA in arteries from the STZ-treated Bmal1 flox/flox and Bmal1 Δ/Δ mice were determined by immunofluorescent staining of consecutive 7-μm aortic sections using specific primary antibodies (Runx2, MBL Cat# D130–3 and SMA, Sigma Cat# A5228). .. To assess a direct regulation of Bmal1 on Runx2 transcription, a 1,974-bp mouse Runx2 promoter (mm10: chr17:44,735,972–44,737,945) containing putative Bmal1 binding sites was insert into the Article Title: Targeted protein O-GlcNAc reveals transcriptional functions for O-GlcNAc. Article Snippet: Technology Targeted protein O-GlcNAc reveals transcriptional functions for O-GlcNAc Article Title: β-catenin: A crucial transcriptional activator of KSHV latency genes and small molecule target in primary effusion lymphoma. Article Snippet: Latency-associated nuclear antigen (LANA)-induced β-catenin accumulation is one of the prime events in the Kaposi sarcoma-associated herpesvirus (KSHV)-associated primary effusion lymphoma (PEL).. LANA interacts with GSK3β, which in turn increases β-catenin pool in this lymphoma.. The intrinsic molecular mechanism and the effects of β-catenin accumulation in this lymphoma is still unclear. β-catenin acts as a major transcription factor that regulates a variety of proliferative signaling pathways and promotes oncogenesis. Article Title: FOSL1 Orchestrates Epigenetic Reprogramming of Anaplastic Thyroid Cancer and Suppresses Natural Killer Cell-Mediated Antitumor Immunity Article Snippet: 2 Anaplastic thyroid cancer (ATC) is the most aggressive type of thyroid cancer with 37 survival time of only 7-10 months.. Previous work revealed reduced proportions and 38 cytotoxicity of natural killer (NK) cells in ATC tumor microenvironment (TME).. Here, we 39 investigated the role of super-enhancers (SEs), clusters of adjacent enhancers and drive high 40 expression of genes, in reshaping the TME in ATC. Clone Assay:Article Title: Targeted protein O-GlcNAc reveals transcriptional functions for O-GlcNAc. Article Snippet: Technology Targeted protein O-GlcNAc reveals transcriptional functions for O-GlcNAc Article Title: TFEB coordinates autophagosome biogenesis and ribophagy during starvation via SQSTM1 Article Snippet: .. The promoter region of human SQSTM1 was amplified by PCR from the U2OS genome and cloned into the Article Title: FOSL1 Orchestrates Epigenetic Reprogramming of Anaplastic Thyroid Cancer and Suppresses Natural Killer Cell-Mediated Antitumor Immunity Article Snippet: 2 Anaplastic thyroid cancer (ATC) is the most aggressive type of thyroid cancer with 37 survival time of only 7-10 months.. Previous work revealed reduced proportions and 38 cytotoxicity of natural killer (NK) cells in ATC tumor microenvironment (TME).. Here, we 39 investigated the role of super-enhancers (SEs), clusters of adjacent enhancers and drive high 40 expression of genes, in reshaping the TME in ATC. Article Title: GAL3ST1-Mediated Histone Tyrosine Sulfation Induced by Cancer-Associated Fibroblasts Promotes Gastric Cancer Metastasis Article Snippet: .. 180 Briefly, GAL3ST1 promoter fragments were amplified and cloned into the Control:Article Title: β-catenin: A crucial transcriptional activator of KSHV latency genes and small molecule target in primary effusion lymphoma. Article Snippet: Latency-associated nuclear antigen (LANA)-induced β-catenin accumulation is one of the prime events in the Kaposi sarcoma-associated herpesvirus (KSHV)-associated primary effusion lymphoma (PEL).. LANA interacts with GSK3β, which in turn increases β-catenin pool in this lymphoma.. The intrinsic molecular mechanism and the effects of β-catenin accumulation in this lymphoma is still unclear. β-catenin acts as a major transcription factor that regulates a variety of proliferative signaling pathways and promotes oncogenesis. Amplification:Article Title: TFEB coordinates autophagosome biogenesis and ribophagy during starvation via SQSTM1 Article Snippet: .. The promoter region of human SQSTM1 was amplified by PCR from the U2OS genome and cloned into the Article Title: GAL3ST1-Mediated Histone Tyrosine Sulfation Induced by Cancer-Associated Fibroblasts Promotes Gastric Cancer Metastasis Article Snippet: .. 180 Briefly, GAL3ST1 promoter fragments were amplified and cloned into the Polymerase Chain Reaction:Article Title: TFEB coordinates autophagosome biogenesis and ribophagy during starvation via SQSTM1 Article Snippet: .. The promoter region of human SQSTM1 was amplified by PCR from the U2OS genome and cloned into the Article Title: METTL3 Methylation Induces Decay of Endogenous Retroelement Transcripts to Promote Tumor Immune Evasion Article Snippet: The genes of SMYD1-5, 177 SETD7, SETD8 were inserted into pcDNA3.1(+) vector (RRID:Addgene_52535). .. The 178 SETD1A and E2F4 promoter region, spanning nucleotides −2000 to +100 relative to 179 the transcription start site (TSS), was PCR-amplified and ligated into the Luciferase:Article Title: TFEB coordinates autophagosome biogenesis and ribophagy during starvation via SQSTM1 Article Snippet: .. The promoter region of human SQSTM1 was amplified by PCR from the U2OS genome and cloned into the Article Title: FOSL1 Orchestrates Epigenetic Reprogramming of Anaplastic Thyroid Cancer and Suppresses Natural Killer Cell-Mediated Antitumor Immunity Article Snippet: 2 Anaplastic thyroid cancer (ATC) is the most aggressive type of thyroid cancer with 37 survival time of only 7-10 months.. Previous work revealed reduced proportions and 38 cytotoxicity of natural killer (NK) cells in ATC tumor microenvironment (TME).. Here, we 39 investigated the role of super-enhancers (SEs), clusters of adjacent enhancers and drive high 40 expression of genes, in reshaping the TME in ATC. Article Title: The Interaction of CircESR1 and HNRNPAB Regulates Cell Cycle Transition of Breast Cancer Cell Article Snippet: .. For transcription factor mediated HNRNPAB expression, 0.2 μg Article Title: METTL3 Methylation Induces Decay of Endogenous Retroelement Transcripts to Promote Tumor Immune Evasion Article Snippet: The genes of SMYD1-5, 177 SETD7, SETD8 were inserted into pcDNA3.1(+) vector (RRID:Addgene_52535). .. The 178 SETD1A and E2F4 promoter region, spanning nucleotides −2000 to +100 relative to 179 the transcription start site (TSS), was PCR-amplified and ligated into the Expressing:Article Title: The Interaction of CircESR1 and HNRNPAB Regulates Cell Cycle Transition of Breast Cancer Cell Article Snippet: .. For transcription factor mediated HNRNPAB expression, 0.2 μg Transfection:Article Title: The Interaction of CircESR1 and HNRNPAB Regulates Cell Cycle Transition of Breast Cancer Cell Article Snippet: .. For transcription factor mediated HNRNPAB expression, 0.2 μg |
